Figure 1. Representative 2D fluorescence difference gel electrophoresis image from sickle cell membrane proteomics study.

Two different fluorescent dyes were used – Cy3 for normal healthy controls and Cy5 for sickle cell patients. The above image is the Cy3 image of the gel, showing spots from normal healthy control samples. Spots outlined in red represent spots that were decreased in sickle cell disease; those that were increased in sickle cell disease are outlined in blue.

Reproduced with permission from [43].