Fig. 3.

Heat map of expression data. Data obtained under control conditions and with externally applied shear forces were combined in a heat map. All data are depicted as log₂ expression values relative to the same set of reference genes (as described in the text) within the range −12 (green) to 14 (red). Genes are grouped by hierarchical clustering (using the Euclidean distance as a distance measure). Values are shown for 6 control experiments with cells grown under static conditions (C), for 3 experiments where cells were exposed to 5 dyn/cm² (L) and 3 experiments where cells were exposed to 15 dyn/cm² (H) shear for 24 h. The data shown here represents the global dataset (three biological repeats for the two flow conditions, with time-matched static conditions for each of these experiments), which was used to construct figures 1, 2 and 4. The gray boxes represent missing data due to failed PCRs, which predictably mostly occurred when gene expression levels were low. Colors refer to the online version only.