Table 1.
Ion channel sub unit expression with two flow-induced shear conditions
| Subunits affected by 5 dyn/cm2 | Subunits affected by 15 dyn/cm2 | Subunits not affected by flow-induced shear | |||
|---|---|---|---|---|---|
| Cav1.2 | Kvl.l | Cav1.2 | Kir6.2 | Cavl.l | Kvβ2 |
| CLC-3 | Kv1.5 | Cav3.1 | Kvl.l | Cav1.3 | M1RP2 |
| CX37 | Kv3.4 | CLC-3 | Kv1.5 | Cav3.2 | P2X4 |
| HCN2 | Kv4.1 | CLC-7 | Kv3.4 | CLC-4 | STIM1 |
| KCa2.2 | ORAI1 | CX37 | Kv4.1 | CX40 | SUR1 |
| KCa2.3 | PMCA1 | CX43 | Kv9.3 | Kca1.1 | SUR2 |
| KCa3.1 | TRPM3 | HCN2 | ORAI1 | KCa4.2 | TMEM16A |
| Kir2.4 | TRPM7 | HCN4 | PMCA1 | Kir2.1 | TRPC1 |
| Kir6.2 | TRPV1 | KCa2.1 | SERCA2 | Kir6.1 | TRPC3 |
| NCX1 | Kca2.2 | TRPM3 | Kvll.l | TRPC4 | |
| KCa2.3 | TRPM4 | Kv7.3 | TRPM1 | ||
| KCa3.1 | TRPM7 | Kvβl | TRPM2 | ||
| Kir2.2 | TRPV1 | ||||
| Kir2.3 | TRPV2 | ||||
| Kir2.4 | TRPV4 | ||||
We performed a SAM test to determine genes regulated by flow: 18 subunits were statistically upregulated and one (NCX1) downregulated by 5 dyn/cm2 shear. A total of 30 subunits were upregulated by 15 dyn/cm2 shear. About 44% of the subunits (24/54) were not significantly regulated by either of the shear levels.