TABLE 1.
Sequence types C and T found in human faecal clones and the respective TaqMan® MGB probes for quantification
Name | Sequence * |
---|---|
Sequence Type C | 5′–CAGGATGGGATCATGA–3′ |
Probe BacH_pC | 3′–MGB–NFQ–GTCCTACCCTAGTACT–FAM–5′ |
Sequence Type T | 5′–CAAGATGGCATCATGA–3′ |
Probe BacH_pT | 3′–MGB–NFQ–GTTCTACCGTAGTACT–FAM–5′ |
Highlighted bases, substitutions in the probe binding sites.