TABLE 2.
Specificity of the BacH q PCR assay tested on human and animal faecal samples and cesspits and waste water samples
| Source | Sample type * | Number of samples ** |
BacH positive/negative |
|---|---|---|---|
| Cesspits and wastewater *** | Wastewater | 20 | 20/0 |
| Human**** | single | 21 | 20/1 |
| Cattle | pooled | 6 (60) | 0/6 |
| Deer | pooled | 4 (40) | 0/4 |
| Chamois | pooled | 4 (40) | 0/4 |
| Roe deer | pooled | 1 (10) | 0/1 |
| Sheep | pooled | 3 (30) | 0/3 |
| Goat | pooled | 3 (30) | 0/3 |
| Horse | pooled | 3 (30) | 0/3 |
| Fox | single | 2 | 0/2 |
| Dog | single | 3 | 0/3 |
| Cat | single | 3 | 1/2 |
| Pig | pooled | 2 (20) | 0/2 |
| Chicken | pooled | 1 (10) | 0/1 |
| Turkey | pooled | 1 (10) | 0/1 |
| Swan | single | 1 | 0/1 |
| Duck | pooled | 1 (10) | 0/1 |
| Black grouse | single | 3 | 0/3 |
single, single faecal sample; pooled, sample pooled from 10 single faecal samples of the respective source or waste water sample;
original number of samples before pooling in brackets;
cesspits from mountain huts in the study area with 10 to 100 inhabitants, municipal waste water from settlements with 300 to 2 million inhabitants from all over Eastern Austria;
persons from Eastern Austria.