Fig. 2.

NleH prevents nuclear condensation induced by TcdB. HeLa cells were infected with wild-type EPEC, EPEC ΔnleH1ΔnleH2 and complemented mutant and stained with Hoechst 33342 for evaluation of nuclear condensation or fragmentation; uninfected and EPEC ΔescN-infected cells were used as controls (a). The number of condensed nuclei (arrowed in a) was determined by counting under an epifluorescence microscope (b).