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. 2010 Jul;156(Pt 7):1953–1962. doi: 10.1099/mic.0.038091-0

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Copyright © 2010, SGM

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — NanoLC-ES-MS/MS analysis of the tryptic digest of C. jejuni 11168 flagellin. The spectra show the glycopeptide ⁴⁶³TTAFGVK⁴⁶⁹ modified with a single glycan. (a) The doubly charged ion at m/z 557.3 derived from the NCTC 11168 Cj1295 : : aphA cmCj1295 strain expressing full-length Cj1295 with an interrupted homopolymeric tract. The characteristic oxonium ion at m/z 391 for the dimethylglyceric acid analogue of pseudaminic acid is underlined. (b) The doubly charged ion at m/z 520.3 derived from the NCTC 11168 Cj1295 : : aphA strain that does not express the Cj1295 gene product. The characteristic oxonium ion at m/z 317 for pseudaminic acid is underlined.