Table 1.
Bacterial strains and plasmids
Y. pestis strains marked with ‘+’ carry the wild-type 102 kb pgm locus, while those without ‘+’ have one or more mutations, or a deletion, in the pgm locus. Hms− indicates the presence of an intact pgm locus, but with deletions in the hmsHFRS, hmsT or hmsP operons; these render the strain incapable of producing wild-type levels of biofilm. HmsC indicates an intact pgm locus coupled with the loss of hmsP; this allows for temperature-independent expression of biofilm.
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| KIM6+ | Pgm+ Lcr− Pla+; pPCP1, pMT1 | Fetherston et al. (1992) |
| KIM5(pCD1Ap)+ | Apr Pgm+, pPCP1+, pMT+, pCD1Ap (′yadA : : bla); derived from KIM6+ | Gong et al. (2001) |
| KIM6-2051.6+ | Hms− (ΔhmsT); Lcr− Pla+; pPCP1, pMT1 | This study |
| KIM5-2051.6(pCD1Ap)+ | Apr Hms− (ΔhmsT2090.2), pPCP1, pMT1, pCD1Ap (′yadA : : bla); derived from KIM6-2051.6 | This study |
| KIM6-2090.1+ | Cmr Hmsc (ΔhmsP : : cam2090.1) Lcr− Pla+; pPCP1, pMT1 | Kirillina et al. (2004) |
| KIM6-2090.2+ | Hmsc (ΔhmsP : : cam2090.1) Lcr− Pla+; pPCP1, pMT1; derived from KIM6-2090.1+ | This study |
| KIM6-2090.7+ | Hmsc, ΔhmsP, pPST+, pMT+, pCD−, Kmr; derived from KIM6-2090.2+ | This study |
| KIM5-2090.7(pCD1Ap)+ | Kmr Apr Hmsc (ΔhmsP2090.2) Δy2360 : : kan2093.1, pPCP1, pMT1, pCD1Ap (′yadA : : bla); derived from KIM6-2090.7 | This study |
| KIM6-2118 | Hms− (in-frame ΔhmsR2118) Lcr− Pla+; pPCP1, pMT1 | Forman et al. (2006) |
| KIM5-2118(pCD1Ap) | Apr Hms− (in-frame ΔhmsR2118) Lcr+ Pla+; pPCP1, pMT1, pCD1Ap (′yadA : : bla); derived from KIM6-2118 | This study |
| Plasmids | ||
| pKD4 | 3.3 kb, Kmr, template plasmid | Datsenko & Wanner (2000) |
| pKD46 | 6.3 kb, Apr, Red recombinase expression plasmid | Datsenko & Wanner (2000) |
| pCP20 | Suicide plasmid with temperature-sensitive replication and thermally induced expression of FLP recombinase; Apr | Datsenko & Wanner (2000) |
| pCD1Ap | 70.5 kb pCD1 with bla cassette inserted into ′yadA; 71.7 kb Lcr+ Apr | Gong et al. (2001) |