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Western blot analysis of HMWP2 and YbtE expression in Y. pestis strains in the presence and absence of exogenous Ybt. Equal concentrations of whole-cell lysates were separated by SDS-PAGE; immunoblots were reacted with antiserum against HMWP2 or YbtE, as indicated. Strains: Ybt+, KIM6+; Δirp2 (in-frame), KIM6-2046.3; irp2ΔS52, KIM6-2046.7; ybtS : : kan, KIM6-2070.1; irp2 : : kan (polar), KIM6-2046.1.
