Table 2.
Primers used in this study
| Primer | Sequence (5′–3′) | Purpose |
|---|---|---|
| ΔirpS | CCAGGCCGGCCTGGATTCC | Check irp2ΔS52 deletion |
| G92A forward | GCTTTTACTCGCCGCGCACAGCATGGGGG | Construction YbtT-G92A-H6 |
| G92A reverse | CCCCCATGCTGTGCGCGGCGAGTAAAAGC | Construction YbtT-G92A-H6 |
| G96A forward | CGGGCACAGCATGGCGGCGCAGGTGGCG | Construction YbtT-G96A-H6 |
| G96A reverse | CGCCACCTGCGCCGCCATGCTGTGCCCG | Construction YbtT-G96A-H6 |
| S94A forward | CGCCGGGCACGCCATGGGGGCGCAGG | Construction YbtT-S94A-H6 |
| S94A reverse | CCTGCGCCCCCATGGCGTGCCCGGCG | Construction YbtT-S94A-H6 |
| H230A forward | GACGGCGATGCTTTCTATCCCATTCAACAAGC | Construction YbtT-H230A-H6 |
| H230A reverse | GCTTGTTGAATGGGATAGAAAGCATCGCCGTC | Construction YbtT-H230A-H6 |
| IHS-1 | AGAAACTGCCGAAATGGTGAGG | Clone y2336 and check y2336 deletion |
| IHS-2 | GAGAGGGAAGCAAACTGCGTATT | Clone y2336 |
| IHS-3 | TTCCCTGGAGATGTTCCCGT | Check y2336 deletion |
| S1 | CGGGATCCGCATGCACGGCGGCTTCAG | Construct irp2ΔS52 deletion |
| S2 | ATCCAGGCCGGCCTGGATCAG | Construct irp2ΔS52 deletion |
| S3 | ATAAGATTGATGAGATGGTTAC | Construct irp2ΔS52 deletion |
| S4 | GCTCTAGATTCACGTAGCGTGGCGGGTTC | Construct and check irp2ΔS52 deletion |
| YbtA-2 | GGGGTACCGACCTGGTTATCTCCCTG | Clone ybtP promoter |
| YbtP-2 | GGGGTACCGGGAGTAACTGAATTTCC | Clone ybtP promoter |
| YbtT For2 | GGCACTGCTGGCGAACGAG | Sequence ybtT mutants |
| YbtT Rev1 | GCGAGTCGTAGTGATAGC | Sequence ybtT mutants |
| YbtT Rev2 | CTTTCTGAAGTACTGGGCTG | Sequence ybtT mutants |