Table 2.

Plasmid constructs

Plasmid	Primer or fragment used*	Construction†
pLJH1 (rsbT1)	T-1 5′ TGCGGATCCATTCCAGGAAGATTTGC 3′	pKSV7 BamHI–PstI
	T-2 5′ GGACCTTTGTTTTTGGCAAC 3′
	T-3 5′ GTTGCCAAAAACAAAGGTCC 3′
	T-4 5′ TTCCTGCAGAGCAATTTCACTACGTA 3′
pLJH2 (rsbUΔ1)	U-1 5′ CTCGGATCCACGTTTAGCGGAGTAC 3′	pKSV7 BamHI–PstI
	U-2 5′ CACTTCTTCTTATTTAGTTTTTTCCATTTTTTTCTAGCT 3′
	U-3 5′ ACTAAATAAGAAGAAGTGAAG 3′
	U-4 5′ AGGCTGCAGAGGAAGTTGTAATGTAA 3′
pLJH3	F 5′ CTTCCTGCAGAATGTAACACTGTCTTT 3′	pPL2 PstI–BamHI
	R 5′ TTTGGATCCTTTATAAGTCTTCGTTAC 3′
pLJH4	BglII–BamHI from pHK77, encoding spoVG–lacZ fusion	pLJH3 SacI–BamHI
pLJH5 (rsbRA)	F 5′ GAAAGGATCCGATGTATAAAGATTTTGCA 3′	pET-15b BamHI–BlpI
	R 5′ CCCGCTCAGCTCACCCCTCTTTTTCTAC 3′
pLJH6 (rsbS)	F 5′ GGTGGATCCTGTGGGGATACCAATCTTA 3′	pET-15b BamHI–BlpI
	R 5′ GAATGCTCAGCTCATTCCCCCAATTCCTG 3′
pLJH7 (rsbT)	F 5′ GGGGGATCCCATGACATTCCAATCCTGT 3′	pET-15b BamHI–BlpI
	R 5′ CTAGCTCAGCTTACCGAACCCATTTCGT 3′

*Restriction endonuclease sites used for cloning are denoted by underlined italic type.

†Vector and sites used: pKSV7 (Smith & Youngman, 1992); pPL2 (Lauer et al., 2002); pET15b (Novagen).