Fig. 5.

Role of PknK in modulation of host immunity. The IL-12p40, TNF-α, IFN-γ and iNOS mRNA levels in mice infected with LIX11 or H37Rv were determined by qRT-PCR over 3 weeks post-infection in RNA extracted from lung tissues pooled from four infected mice in each group. Normalization of expression was done with β-actin mRNA. qRT-PCR analysis was done in triplicate and data are presented as means±sd from at least two independent cDNA preparations. ** and *** denote P<0.01 and P<0.001, respectively, for the differences in the expression of IL-12p40, TNF-α, IFN-γ and iNOS in the mutant-infected animals versus the H37Rv-infected mice at a given time point.