Table 5.

Phenotypes of NarL missense mutants

narL allele*	Percentage of wild-type regulation† in strain‡
	Repression		Class I activation		Class II activation
	O1-nrfA	O1-napF	Φ(narG–lacZ)	Φ(fdnG–lacZ)	Φ(napFEc–lacZ)	Φ(napFHi–lacZ)
None	0	0	0	0	0	0
Wild-type	100	100	100	100	100	100
Deficient
L171A	85	68	1.2	24	48	56
K199A	94	86	1.0	26	40	43
Functional
D162A	96	97	70	52	52	86
M175A	100	101	86	92	103	185
Q169A	99	102	98	96	57	313
L166A	98	99	119	85	70	242
Positive control
R178A	101	99	18	33	86	86
R179A	100	101	61	52	226	100
D181A	98	102	52	41	146	93
R178A+R179A	101	105	<0.1	5.5	8	28
R179A+D181A	101	81	39	14	20	31

*Indicated allele integrated at att Φ80.

†Percentage repression or activation relative to wild-type value during growth with nitrate (Zhang et al., 1992).

‡VJS10461 (O1-nrfA), VJS9719 (O1-napF), VJS10649 [λΦ(napF–lacZ)], VJS10247 [λΦ(napF_Hi–lacZ)], VJS10258 [λΦ(narG–lacZ)] and VJS10248 [λΦ(fdnG–lacZ)].