Table 1.
Strains and plasmids
| Strains | Lab DH no. | Description | Source |
|---|---|---|---|
| P. aeruginosa | |||
| PA14 | 122 | Wild-type | Rahme et al. (1995) |
| PA14ΔlasR | 164 | In-frame deletion of lasR | Hogan et al. (2004) |
| PA14ΔlasI | 132 | In-frame deletion of lasI | Hogan et al. (2004) |
| PA14 rhlR : : TetR | 6 | Gene replacement of rhlR | Hogan & Kolter (2002) |
| PA14ΔrhlI | 169 | In-frame deletion of rhlI | Hogan et al. (2004) |
| PA14ΔlasRrhlR : : TetR | 237 | In-frame deletion of lasR in DH6 | This study |
| PA14ΔlasRΔrhlI | 238 | In-frame deletion of lasR in DH169 | This study |
| PA14ΔpqsH | 1112 | In-frame deletion of pqsH | This study |
| PA14ΔlasRΔpqsH | 1113 | In-frame deletion of lasR in DH1112 | This study |
| PA14ΔpqsR | 1110 | In-frame deletion of pqsR | This study |
| PA14ΔlasRΔpqsR | 1111 | In-frame deletion of lasR in DH1110 | This study |
| CIA467G | 1097 | LasR mutation, 467 nt mutated, A→G, nonsynonomous D→G | Smith et al. (2006) |
| CIG691C | 1099 | LasR mutation, 691 nt mutated, G→C, nonsynonomous A→P | Smith et al. (2006) |
| CIT341C | 1100 | LasR mutation, 341 nt mutated, T→C, nonsynonomous L→P | Smith et al. (2006) |
| CIG608A | 1103 | LasR mutation, 608 nt mutated, G→A, nonsynonomous C→Y | Smith et al. (2006) |
| CIG179A | 1132 | LasR mutation, 179 nt mutated, G→A, nonsynonomous W→STOP | Smith et al. (2006) |
| CIC181T | 1134 | LasR mutation, 181 nt mutated, C→T, nonsynonomous R→C | Smith et al. (2006) |
| CIG455- | 1136 | LasR mutation, 455 nt deleted, G→ – | Smith et al. (2006) |
| E. coli | |||
| S17/λpir | 71 | Laboratory collection | |
| DH5α | – | F′/endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 (lacZYA-argF) U169 deoR [80 dlac (lacZ)M15 recA1] | Laboratory collection |
| JM109 | 1406 | F′ traD36 proA+B+ lacIq Δ(lacZ)M15/Δ (lac-proAB) glnV44 e14− gyrA96 recA1 relA1 endA1 thi hsdR17 | Laboratory collection |
| C. albicans | |||
| SC5314 | 35 | Wild-type | Gillum et al. (1984) |
| S. cerevisiae | |||
| DC49-7.1C | – | MATα, leu2-3, 112 trp1-289 ura3-52 arg4− Δ57, RV | Shanks et al. (2006) |
| Plasmids | |||
| pUCP22 | 160 | GmR; Escherichia–Pseudomonas expression plasmid | West et al. (1994) |
| pEX18GM | GmR; oriTsacB, gene replacement vector with MCS from pUC18 | Hoang et al. (1998) | |
| pMQ30 | 962 | Deletion plasmid for yeast cloning | Shanks et al. (2006) |
| pLASR | 133 | PA14ΔlasR deletion construct | Hogan et al. (2004) |
| pPQSRdel | 949 | PA14ΔpqsR deletion construct | This study |
| pPQSHdel | 1109 | PA14ΔpqsH deletion construct | This study |
| pCR2.1TOPO | – | Cloning vector | Invitrogen |
| pPQSHFLAG | 1413 | pqsH promoter region and gene in pUCP22 | This study |
| pSB536 | 1407 | ahyR+ ahyI : : luxCDABE; AmpRcolE1 origin | Swift et al. (1997) |