Table 1.
Kinetic properties of ADH enzymes in the parent T. vaginalis SS22, T. vaginalis infected with M. hominis (SS22-MOZ2) and M. hominis MOZ2
Enzyme assays were performed as described in Methods using whole-cell extracts. Vmax is expressed as μM min−1 using 1 mg protein. The Vmax and Km values are expressed as mean±sd for four determinations. nd, Not detected.
| Enzyme | T. vaginalis SS22 | T. vaginalis SS22-MOZ2 | M. hominis | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Vmax (μM min−1) | Km (μM) | Vmax/Km | Vmax (μM min−1) | Km (μM) | Vmax/Km | Vmax (μM min−1) | Km (μM) | Vmax/Km | |
| ADI | 76.6±7.4 | 200±36 | 0.38 | 433±14.0 | 60±10 | 7.2 | 516±24 | 60±7.1 | 8.6 |
| cOCT | 40.1±4.5 | 138±18 | 0.29 | 48.0±12.1 | 125±23 | 0.38 | 11864±432 | 180±12 | 65.9 |
| aOCT | 1938±210 | 3400±170 | 0.57 | 1624±180 | 2900±240 | 0.56 | 75964±9258 | 4200±295 | 18.1 |
| CK | 3900±480 | 60±3.8 | 65.0 | 4274±390. | 83±12.0 | 51.5 | 1274±94 | 95±12 | 13.4 |
| ODC | 1.10±0.2 | 18±3.0 | 0.06 | 1.15±0.3 | 16±2.0 | 0.07 | nd | nd | – |