Table 1.
Action Potential Characteristics | Ca2+ Transient Kinetics | ||||||
---|---|---|---|---|---|---|---|
Cell Type | RMP (mV) | APA (mV) | APD50 (ms) | APD90 (ms) | Time to Max (ms) | Peak Rate of Rise (F/Fo/s) | Tau of decay (ms) |
EGFP(−) (n=10) | −71.3±1.1 | 119.2±3.2 | 5.5±0.9 | 54.7±9.7 | 29.5±3.6 | 103.0±20 | 110.4±13.4 |
EGFP(+) (n=10) | −71.5±1.3 | 128.3±3.7 | 24.7±4.3 | 139.3±17.8 | 49.4±4.8 | 49.4±5.9 | 136.7±23.2 |
P value | .87 | .08 | .00035 | .00057 | < .000001 | < .00001 | 0.02 |
Data are expressed as mean values ± SEM. N, number of cells; RMP, resting membrane potential; APA, AP amplitude; APD50,90, AP duration at 50% and 90% of repolarization, respectively. APDs are significantly longer for Cntn2-EGFP+ myocytes compared to Cntn2-EGFP− myocytes. Ca2+ transient measurements demonstrated that Cntn2-EGFP+ myocytes have significantly slower Ca2+-handling kinetics compared to Cntn2-EGFP− myocytes.