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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Dev Cell. 2011 Mar 15;20(3):329–341. doi: 10.1016/j.devcel.2011.01.007

Figure 5. Mutations in DivK that affect DivL binding in vitro affect CckA activity in vivo.

Figure 5

(A) In vitro FRET analysis of DivL-YFP binding to wild type DivK-CFP and the mutants indicated. Assays were performed as in Fig. 5A. Wild-type and free CFP/YFP traces are the same experiments as in Fig. 5A and are duplicated to facilitate comparison. (B) Phase contrast microscopy and flow cytometry analysis of strains expressing either divK or divK(Q55A) from a low-copy plasmid under the control of a xylose-inducible promoter. Cells were grown in glucose or in the presence of xylose for 6 hours. (C) In vivo phosphorylation measurements of CckA in a mixed population of cells expressing divK or divK(Q55A). Assays were performed as in Fig. 2A, except strains were induced with xylose for 2 hours and compared to identically treated, but uninduced cultures. Error bars represent standard deviations from three independent replicates.