The S1P2 receptor regulates macrophage recruitment. S1p2r+/+ and S1p2r−/− mice were injected i.p. with TG. Five or 15 d later, cells were collected by peritoneal lavage and analyzed for macrophage content. A, Flow cytometric analysis of peritoneal cells on days 5 and 15, showing percentage of cells that are CD11b+F480+, and CD11c expression in this population; (day 5 S1p2r+/+ n = 14, S1p2r−/− n = 11) (day 15 S1p2r+/+ n = 10, S1p2r−/− n = 9). B, Absolute numbers of peritoneal macrophages, polymorphonuclear neutrophils, and lymphocytes recovered from the peritoneum on day 5 and 15. C, Flow cytometric analysis of draining LNs and nondraining LNs on day 15, showing percentage of cells that are CD11b+F480+.