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S1P2R selectively inhibits chemoattractant-induced Akt phosphorylation. S1p2r+/+ and S1p2r−/− BMDM were analyzed by Western blot for protein phosphorylation. A, Phospho-Akt and phospho-ERK analysis after 5-min stimulation with 10 nM C5a, with or without 100 nM S1P. B, Time-course of phospho-PAK1 analysis in S1p2r+/+ BMDM stimulated with 10 nM C5a, with or without 100 nM S1P.
