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. Author manuscript; available in PMC: 2011 Mar 31.
Published in final edited form as: J Immunol. 2009 Dec 30;184(3):1475–1483. doi: 10.4049/jimmunol.0901586

FIGURE 6.

FIGURE 6

PTEN is not required for S1P2R-mediated inhibition. A, PtenloxP/loxP, Cre ERT2, and PtenloxP/loxP mice were fed tamoxifen for 5 d and bone marrow was cultured to generate PtenΔ/Δ and Pten+/+ BMDM, respectively. Inset shows immunoblot analysis of BMDM extracts for respective Abs. B, In vitro migration of PtenΔ/Δ and Pten+/+ BMDM toward 10 nM C5a, with or without 10 nM S1P, was measured using a modified Boyden chamber. C, Western blot analysis of phospho-Akt in PtenΔ/Δ and Pten+/+ BMDM stimulated with 10 nM C5a, with or without 100 nM S1P.