Figure 3.

Suppressive effects of ATF3 on Tax-mediated transactivation through ATF/CRE sites. Jurkat cells were cotransfected with phRL-TK and expression vectors for ATF3, HBZ, and reporter plasmid pCRE × 4-luc (A), WT-luc (B), or LTR-luc (C) respectively. The total amount of DNA for transfection was equalized by adding empty vectors. After 24 hours, a dual luciferase reporter assay was performed as described in Materials and Methods. All the data are relative values of firefly luciferase normalized to Renilla luciferase and shown as a mean of a triplicate set of experiments (mean ± SD). *P <0.05; **P <0.01.