Table 1.

Comparison of EDIII-based monovalent antigen mixture and single tetravalent antigen in anti-DENV antibody detection^a

	IgG (n = 80)			IgM (n = 81)
Parameter	EDIII Mix	EDIII-T	Mean Ratio of S/Co values (95% CI)b	EDIII Mix	EDIII-T	Mean Ratio of S/Co values (95% CI)b
% Sensitivityc	81.3	82.5	1.22 (1.15-1.29)	59.3	63.0	1.11 (1.06-1.16)
% Specificityd	100	100	-	100	100	-

^aAssays were done using polystyrene microtiter wells that were coated either with a mixture of four EDIII monovalent antigens (EDIII Mix) or the single tetravalent antigen, EDIII-T. The number of sera analyzed is denoted by n.

^bThis column represents the mean ratio of S/Co values using EDIII-T to S/Co values using EDIII Mix as the coating antigens; the cut-off values were 0.30 and 0.33 for EDIII mix and EDIII-T, respectively, in the IgG assay, and 0.23 and 0.25, respectively, in the IgM assay. The 95% confidence interval is indicated in parentheses.

^cPercent Sensitivity = (Number of sera that scored positive by our test/Number of sera that scored positive using PanBio ELISAs) × 100

^dPercent Specificity = (Number of sera that scored negative by our test/Number of sera that scored negative using PanBio ELISAs) × 100