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. 2011 Mar 15;11:64. doi: 10.1186/1471-2334-11-64

Table 2.

The performance of b-EDIII-T as a capture antigen in the detection of anti-DENV antibodiesa

IgG (n = 80) IgM (n = 81)


Parameter PS SA Mean Ratio of S/Co
values (95% CI)b
PS SA Mean Ratio of S/Co
values (95% CI)b
% Sensitivityc 82.5 91.3 1.32 (1.24-1.39) 61.7 75.3 1.30 (1.23-1.37)

% Specificityd 100 100 - 100 100 -

aAssays were done using polystyrene (PS) microtiter wells or streptavidin (SA) coated microtiter wells. The number of sera analyzed is denoted by n.

bThis column represents the mean ratio of S/Co values using SA plates to S/Co values using PS plates; the cut-off values were 0.31 and 0.28 for PS and SA, respectively, in the IgG assay, and 0.23 and 0.25, respectively, in the IgM assay. The 95% confidence interval is indicated in parentheses.

cPercent Sensitivity = (Number of sera that scored positive by our test/Number of sera that scored positive using PanBio ELISAs) × 100

dPercent Specificity = (Number of sera that scored negative by our test/Number of sera that scored negative using PanBio ELISAs) × 100