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. 2011 Mar 17;6:6. doi: 10.1186/1745-6673-6-6

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Copyright ©2011 Wisnewski et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Detection and fractionation of the major MDI antigen in detergent extracts of exposed skin. (A) Proteins from (-) control or (+) 1% MDI exposed mouse skin, were separated by SDS-PAGE and stained with commassie blue or Western blotted with autologous sera from MDI skin exposed mice (lanes 3 and 4) or control mice (lanes 5 and 6). Arrow highlights major antigenic protein from exposed skin, with apparent shift in migration, indicating change in conformation/charge. (B) The MDI antigen, highlighted by arrows, was separated from other skin proteins by isoelectric focusing. Shown is Ponceau S protein staining of Rotofor^®fractions 2-16 after SDS-PAGE and transfer to nitrocellulose membrane. Lanes 1 and 17 contain prestained molecular weight markers.