Skip to main content
. 2011 Mar 31;7(3):e1001329. doi: 10.1371/journal.ppat.1001329

Figure 7. The DYNA-IND IAV entry pathway is EIPA sensitive.

Figure 7

The effect of 80 µM EIPA on DYNA-DEP (A) or DYNA-IND (B) entry was examined in the Gluc-entry assay (HeLa cells; strain WSN; MOI 0.5; incubation with EIPA from −1 hr to 2 hr p.i.). Data were plotted relative to the control (0.2% DMSO). (C) Redundancy of DYNA-DEP and DYNA-IND entry pathways in 10% FCS using the Gluc-entry assay. The effect of 80 µM dynasore (DY; red), 80 µM EIPA (orange) or 80 µM of both inhibitors (DY+EI; green) in the presence of 10% FCS is shown (HeLa cells; strain WSN; MOI 0.5). (D,E) Redundancy of DYNA-DEP and DYNA-IND entry pathways in 10% FCS using the VLP entry assay. VLP entry was performed in Optimem containing 10% serum in the presence of 80 µM dynasore (DY; red), 80 µM EIPA (orange) or 80 µM of both inhibitors (DY+EI; green). Panel E displays the quantified data of the FACS histograms displayed in panel D. (F,G) Virus production was measured by determining infectivity in the supernatant of cells inoculated in the presence of 80 µM dynasore (DY), 80 µM EIPA or 80 µM of both inhibitors (DY+EI) in PBS (panel E) or PBS+10% FCS (panel F). After 2 hr the medium was replaced by growth medium containing 10% FCS and 10 nM BafA1. Supernatant was harvested 24 hr p.i. for TCID50 determination (Y-axis).