Figure 6. Arabidopsis lecrk-I.9 and 35S-ipiO1 lines are phenocopies.
Both lines show (A) gain of susceptibility to P. brassicae, and (B) defects in cell wall integrity. (A) Leaves inoculated with P. brassicae isolate HH 3 days post-inoculation (dpi). (B) Elicitation of plasmolysis in etiolated hypocotyls by treatment with 0.4 M CaCl2. Shown are concave (white arrowheads) and convex (black arrowheads) forms of plasmolysis. In Col-0 only a few cells show convex plasmolysis, in lecrk-I.9 convex shapes occur more frequently whereas in 35S-ipiO1 the majority of cells is convex. Etiolated hypocotyls were stained with neutral red. Scale bars represent 100 µm.