Table 2.

Equilibrium constants for [³H]glibenclamide binding to brain regions from WT and SUR1 Tg mouse lines

Genotype	Constant	Cortex	Striatum	Hippocampus	Cerebellum
WT (n = 8)	Kd, nM	1.2  ± 0.42	1.1  ± 0.17	1.3  ± 0.16	0.9  ± 0.18
	Bmax, pmol/mg protein	0.37  ± 0.04	0.40  ± 0.08	0.38  ± 0.04	0.34  ± 0.04
Tg174 (n = 6)	Kd, nM	1.4  ± 0.16	0.76  ± 0.14	0.69  ± 0.12	0.65  ± 0.11
	Bmax, pmol/mg protein	5.3  ± 0.71*†	2.2  ± 0.36*	1.8  ± 0.22*	0.78  ± 0.08
Tg166 (n = 6)	Kd, nM	1.6  ± 0.36	1.1  ± 0.44	1.1  ± 0.48	0.60  ± 0.09
	Bmax, pmol/mg protein	2.1  ± 0.36*	1.4  ± 0.17	1.5  ± 0.58	0.52  ± 0.14

[³H]Glibenclamide binding to cell membranes from brain regions dissected from WT and Tg mice was assessed by using saturation assays. Values represent the mean ± SEM. The striata and hippocampi from two mice were pooled for each assay. 

^*, Significantly different from WT mice, P < 0.05, ANOVA followed by Tukey's multiple comparison test. 

†, Significantly different from Tg166 mice, P < 0.05, ANOVA followed by Tukey's test.