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. 2011 Apr 1;22(7):1035–1044. doi: 10.1091/mbc.E10-11-0901

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© 2011 Gogendeau et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — PtSas4 overexpression generates supernumerary germinative disks but not longer basal bodies. (A) Electron micrograph of a control cell displaying a duplicating cortical unit. Extra germinative disks are never seen in control cells. (B, C) Electron micrographs of cells overexpressing GFP-PtSas4 (PtSas4OE). In 65% of cortical basal body units from PtSas4OE cells, we identified extra germinative disks. Next to a row of duplicating basal bodies (note the Cr on the top of the two old basal bodies in B and a maturing daughter basal body in C), extra germinative disks can be seen (arrows). Bar = 50 nm. (C′) Enlargement of the germinative disk seen in (C). By modulating the contrast, it is possible to detect a structure that resembles a hub (arrow) inside the germinative disk. Bar = 50 nm. (D) A graph showing measurements of the length of basal bodies from control cells and PtSas4OE cells.