FIGURE 2:

Migration of wild-type FAK, Y925F-FAK, and Y925E-FAK cells. (A) After wounding, migrating cells expressing wild-type or mutant fluorescent FAK (see inserts in t = 0 images) were tracked for 13 h. Panels show representative images of migrating cells at the wound leading edge taken after 4, 8, and 13 h. Fluorescent cells are numbered, and nonfluorescent cells are labeled alphabetically. Scale bar, 20 μm. (B) The average migration speed was quantified from the tracking data for control FAK^−/− (n = 111), wild-type FAK (n = 14), Y925F-FAK (n = 19), and Y925E-FAK (n = 16) cells. *p < 0.0013 vs. wild-type FAK cells, #p < 0.003 and ##p < 0.0002 vs. control cells, ††p < 0.0002 vs. Y925F-FAK cells.