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. 2011 Mar 31;6(3):e17890. doi: 10.1371/journal.pone.0017890

Figure 4. Anti-TNF-α agents promote the expansion of γδ-T cells in IL-2 stimulated PBMNC cultures.

Figure 4

PBMCs were labelled with CFSE and were analysed by flow cytometry after 5 days of culture. γδ-T cells were stained with APC. The anti-TNF-α agents (infliximab, adalimumab, and etanercept) induced proliferation of γδ-T cells in vitro (middle panel), whereas the proliferation on non-γδ-T cells was negligible (right panel). In IL-2 -supplemented (200 IU/ml) PBMC cultures, infliximab (0.1 µg/ml or 1.0 µg/ml) induced a dose-dependent proliferation of γδ-T cells (middle panel). Etanercept induced proliferation of γδ-T cells in the same magnitude.The anti-IL-12p40 antibody ustekinumab had no effect on γδ-T cell proliferation in vitro. PBMCs from three CD patients and two healthy controls were analyzed.