Figure 1. Slow growing and normally developing pre-erythrocytic (PE) forms.

Two types of hepatic parasites can be clearly distinguished from days 5 post-infection onwards, in in vitro cultured human or M. fascicularis primary hepatocytes infected with P. falciparum or P. cynomolgi sporozoites, respectively. The representative photomicrographs were made on cultures fixed on Day 5 and Day 11 post-infection. The parasite and host nuclei are stained with DAPI (blue), while the parasites are labelled by an antibody specific to the HSP70 of the two parasite species (green). Mature PE forms (Mat-PE) and uninucleate small PE forms (PE-uni) are clearly distinguishable.