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. 2011 Mar 31;6(3):e18197. doi: 10.1371/journal.pone.0018197

Figure 2. Cryptic promoter sequence analysis.

Figure 2

(A) The cDNA sequence encoding the 5′ terminal 170 nt of the DENV2 RNA genome. The putative −10 and −35 cryptic promoter elements and the putative Shine-Dalgarno sequences (red) are aligned with their corresponding E. coli wild type elements (blue). The cDNA encoding the authentic DENV2 start codon (97AUG) and the in-frame alternate start codon (139AUG) are underlined. The predicted transcription initiation site is at the cDNA encoding DENV2 nt 87. (B) Fluorescent microscopy images of E. coli cells transformed with deletion mutant plasmids that result in truncations of 50 nt, 67 nt and 85 nt from the 5′ end of DENV2 RNA, respectively.