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. 2011 Mar 31;6(3):e18197. doi: 10.1371/journal.pone.0018197

Figure 3. D2-GFP translation initiates in E coli at an alternate start codon (139AUG) by a Shine-Dalgarno independent mechanism.

Figure 3

(A) Western blots of E. coli (lanes 1 and 2) and BHK (lanes 3 and 4) cell lysates expressing D2-GFP and D2-139T-GFP probed with anti-GFP antibodies. E. coli was transformed with either pT7-D2-GFP or pT7-D2-139T-GFP. BHK cells were transfected with in vitro transcribed D2-GFP and D2-139T-GFP RNA. (B) Fluorescent microscopy images of E. Coli cells transformed with either pT7-D2-GFP or pT7-D2-116TCC-GFP.