Figure 4. The efficiency of the DENV cDNA cryptic bacterial promoter.

E coli cells transformed with pD2-GFP, pDSW207 or pDSW208 were cultured at 37°C for 5h in the absence (lanes 1–4) or presence (lanes 5–8) of IPTG. The numbers of bacterial cells in each culture were normalized, and the cells were pelleted by centrifugation before lysis. Proteins were resolved by SDS-PAGE and analysed by Western blot employing anti-GFP antibodies. The volume of clarified lysate loaded into each lane is shown.