Table 1. Quantitative detection of GFP gene in total cellular RNA extracted from E. coli cells transformed with pD2-GFP, pD2-GFP mutants and pKUN-GFP.

Plasmid	Copies of mRNA*	P value
pD2-GFP	6.6
pΔ50D2-GFP	6.5	>0.05
pΔ67D2-GFP	6.3	>0.05
pΔ85D2-GFP	3.8	<0.01
pD2-74G-GFP	5.7	<0.05
pD2-74GCG-GFP	4.6	<0.01
pKUN-GFP	5.7	<0.05

*Log₁₀ copies of GFP gene/µg of total RNA.