Figure 1. Physical interaction of LRP16 and p65.

(A) GST and GST-LRP16 stained with Coomassie Blue are shown in the left panel. [³⁵S]methionine-labeled UXT, p65, p50 or c-Rel was incubated with GST or GST-LRP16 as indicated in the middle and right panels. (B) The left panel shows a schematic illustration of p65 and its mutants. GST pull-down assays were performed with various [³⁵S]-labeled mutants of p65 and GST-LRP16 or GST alone (right panel). (C) The left panel shows a schematic illustration of LRP16 and its mutants. The middle panel shows GST, GST-LRP16 and its mutants stained with Coomassie Blue. GST pull-down assays were performed with [³⁵S]-labeled p65 and GST-LRP16 mutants or GST alone (right panel). (D) 293T cells were treated with TNF-α (10 ng/ml) for the indicated times. Cell lysates were immunoprecipitated and immunoblotted with the indicated antibodies. (E) 293T cells were cotransfected with p65 and Flag-tagged constructs. Forty-eight hours after transfection, cells were lysed and the lysates subjected to CoIP using the indicated antibodies. The asterisk indicates a non-specific band. NsIgG: non-specific IgG.