Figure 2. Ectopic expression of LRP16 markedly upregulates cytokine-induced κB-luc activity.

(A–D) 293T cells were cotransfected with 3×κB-luc and the indicated vectors. Forty-two hours after transfection, cells were simulated with 10 ng/ml TNF-α or 20 ng/ml IL-1βfor 7 h before luciferase assays were performed. The relative levels of luciferase activity were normalized to the activity obtained after cotransfection of 3×κB-luc and the empty expression vector, which was arbitrarily assigned a value of 1. All experiments were performed in triplicate and were repeated at least three times, and the results are expressed as means±SD. ^* P<0.05.