Figure 3. Ectopic expression of LRP16 does not significantly augment TNF-α-induced expression of the endogenous NF-κB target genes and NF-κB DNA binding capacity.

(A) 293T cells, which were stably transfected with the LRP16 expression construct or empty vector, were treated with TNF-α (10 ng/ml) for the indicated times. Endogenous expression of IκBα and A20 mRNA was measured by qPCR. Data represent means±SD (error bars) of at least three independent experiments. (B) 293T cells, which were stably transfected with the LRP16 expression or empty vector, were treated with TNF-α (10 ng/ml) for the indicated times, lysed and the nuclear extracts subjected to EMSA assays. Sp1 was used as a loading control.