Figure 5. Knockdown of LRP16 sharply attenuates the DNA binding capacity of NF-κB.

(A) 293T or HeLa cells were transfected with the indicated siRNAs. Forty-eight hours after transfection, cells were stimulated with 10 ng/ml TNF-α for the indicated times, lysed and the nuclear extracts subjected to EMSA assays. Sp1 was used as a loading control. (B) 293T cells were treated as in (A). The ChIP assays were performed for the A20, IκBα and GAPDH promoters using the indicated antibodies and corresponding primers described in Materials and Methods.