Figure 7. Knockdown of LRP16 sensitizes TNF-α-induced cell apoptosis.

(A) 293T cells were transfected with the indicated siRNAs. Forty-eight hours later, cells were treated with TNF-α plus CHX or left untreated for 22 h. Representative microscopic images are shown (right panel for DAPI staining). Bar = 20 µm. (B) 293T cells were treated as in (A), the percentage of apoptotic cells was monitored by annexin V staining followed by FACS analysis. Experiments to analyze apoptosis were performed in triplicate and were repeated at least three times, and the results are expressed as means±SD. (C) 293T cells were transfected with the indicated siRNAs. Forty-eight hours after transfection, cells were stimulated with 10 ng/ml TNF-α for an additional 12 h. Total protein was prepared and immunoblotting was performed with the indicated antibodies.