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. 2011 Mar 31;6(3):e18305. doi: 10.1371/journal.pone.0018305

Figure 6. AAA does not inhibit KA-induced loss of RGCs.

Figure 6

C57BL/6 mice were treated by intravitreal injection of PBS, KA (10 nM), AAA (100 ug), and KA plus AAA. At 24, 48, and 72 h after treatment, loss of RGCs was determined by immunofluorescent staining of retinal flat mounts with antibodies against Brn3a (left panel). Immunofluorescent staining and quantification of cell loss (right panel) indicate that while Brn3a-positive RGCs remained similar in PBS and AAA-treated animals, Brn3a-positive RGCs were reduced significantly in animals treated with KA (*, p<0.05) or KA plus AAA (**, p<0.05). All images were acquired at 40× magnification.