Figure 8. Neurostatin alone reduces GFAP expression.

C57BL/6 mice (n = 6) were treated by intravitreal injection of PBS, KA (10 nM), or KA plus Neurostatin (5 mM). At 24, 48 and 72 h after injection, retinal flat mounts were immunostained with antibodies against GFAP (top panel) and retinal cross sections (lower panel) with antibodies against GFAP (green) and vimentin (red fluorescence). Retinal cross sections were also counterstained with DAPI (blue). Immunostaining results indicate that Neurostatin alone reduces GFAP expression in both astrocytes and Mϋller cells. All images were acquired at 40× magnification.