Figure 11. Neurostatin attenuates KA-induced ganglion cell loss.

C57BL/6 mice were treated by intravitreal injection of PBS, KA (10 nM), Neurostatin (5 mM), or KA plus Neurostatin. At 24, 48, and 72 h after the treatment, loss of RGCs was determined by immunofluorescent staining of retinal flat mounts with antibodies against Brn3a (left panel). Immunofluorescent staining and quantification of cell loss (right panel) indicate that while Brn3a-positive RGCs remained similar in both PBS and Neurostatin-treated animals, Brn3a-positive RGCs were decreased significantly in KA-treated retinas (+, p<0.05). In contrast, Brn3a-Positive RGC loss was inhibited significantly (++, P<0.05) in animals treated with KA plus Neurostatin. All images were acquired at 40× magnification.