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. 2011 Mar 31;6(3):e18450. doi: 10.1371/journal.pone.0018450

Figure 1. Circadian oscillation of AMPK activity in hypothalamus across the 24 hr light-dark cycle.

Figure 1

Left, A representative Western blot showing the phosphorylation level of AMPK (T172) in extracts from mouse hypothalamus which were harvested at 4 hr intervals in a 12h light:12h dark (LD) cycle. The entire hypothalamus from 3 months old male C57BL/6J mice was used for this experiment. Right, Quantification of phosphorylated AMPKα (T172) is shown in arbitrary units (n = 3–4 per time point). Average AMPK activity (arbitrary unit) was calculated by densitometric quantification of phosphorylated proteins normalized to total proteins. Lights-on (6am; light) is indicated by a white bar and lights-off (6pm; dark) is indicated by a black bar. Results are means ± S.E. * P<0.05 between 10 am and 10 pm.