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. 2011 Mar 31;6(3):e18450. doi: 10.1371/journal.pone.0018450

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(A) Nampt gene expression patterns of heart, skeletal muscle and fat tissue during 24 hr for AMPKα1−/−, AMPKα2 −/− and WT mice. The relative levels of mRNA are presented in arbitrary units. Results are expressed as mean ± S.E., * P<0.05, # P<0.001 between WT and AMPKα deficient mice. (n = 4–5 for each time point). The same WT data is plotted for α1 KO and α2 KO. (B) Nampt gene expression (arbitrary units) and cellular NAD+ and NADH level in WT and AMPKα1/α2 KO mefs synchronized with forskolin. Results are expressed as mean ± S.E., * P<0.05, between WT mefs and AMPKα1/α2 KO mefs (n = 3).