Table 1. Putative EE–binding factors identified by LC-MS/MS.
| Accession number | Family(Gene name) | Number of protein-specific peptides identified | ||||||
| Replicate 1 | Replicate 2 | Replicate 3 | ||||||
| AGI | Uniprot | EE-wt | EE-mt | EE-wt | EE-mt | EE-wt | EE-mt | |
| At3g09600.1 | Q8RWU3 | Myb-like (RVE8) | 1 | - | 4 | - | 4 | - |
| At5g02840.1 | Q6R0G4 | Myb-like (RVE4) | - | - | - | - | 2 | - |
| At5g47390.1 | Q9LVS0 | Myb-like | 3 | - | - | - | - | - |
| At1g49480.1 | Q9XIB5 | B3 domain(RTV1) | 5 | 2 | - | - | - | - |
| At3g18990.1 | Q8L3W1 | B3 domain (VRN1) | 2 | - | - | - | - | - |
| At4g36730.1 | P42774 | Basic-leucine zipper (GBF1) | 3 | 3 | - | - | - | - |
| At1g76890.1 | Q39117 | Trihelix (GT2) | 2 | - | - | - | - | - |
| At1g76880.1 | Q9C6K3 | Trihelix | - | 2 | - | - | - | - |
| At1g13960.1 | Q9XI90 | WRKY (WRKY4) | - | 4 | - | - | - | - |
| At1G14410.1 | Q9M9S3 | WHIRLY (WHY1) | 14 | 14 | 11 | 9 | 10 | 9 |
| At2g02740.1 | A8MR64 | WHIRLY (WHY3) | 13 | 14 | 9 | 9 | 9 | 6 |
In three independent experiments, plant extracts were incubated with either wild-type or mutant versions of the EE bound to beads. Purified proteins were digested with trypsin and peptides were identified by LC-MS/MS. The number of peptides that could be specifically assigned to a single protein (not shared with any homologous proteins) using a peptide false discovery rate of 0.1% is indicated. Only known and putative transcription factors are listed (169 total proteins were identified). Complete protein identification data and statistics are available from the proteome commons data set as detailed in the methods section. The peptide numbers reported are based on scaffold grouping. For full information see Table S2 or the scaffold file in the proteome commons data repository.