Figure 1.

Activation of TrkA receptors with G protein-coupled receptor ligands. Stably transfected PC12 cells expressing high levels of TrkA (615) were treated with the indicated compounds for 2 h. The cells were subsequently harvested in lysis buffer as described in Materials and Methods. Lysates were immunoprecipitated with anti-pan Trk rabbit antiserum. Immunocomplexes were analyzed by immunoblotting with anti-phosphotyrosine antibody (PY99). Immunoprecipitation of TrkA receptors was then confirmed by immunoblotting of the immunocomplex with anti-pan Trk antiserum.