Figure 1. Synchronization of procyclic cells by starvation.

Four biological replicates were performed in which cells were released from starvation at time t = 0 and samples collected for flow cytometry and RNA isolation at t = 5, 7 and 9 hours post-release. A: Flow cytometry profiles of synchronized procyclic cells. Propidium iodide fluorescence (indicating DNA content) is measured on the x-axes and cell count is plotted on the y-axes. B: Northern hybridization of a known cell-cycle regulated transcript (LIGKA) against RNA from synchronized cells.