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. 2011 Mar 31;7(3):e1001360. doi: 10.1371/journal.pgen.1001360

Figure 5. Nutlin induces TLR2 and TLR5 mRNA expression as well as proteins.

Figure 5

(A) Induction of TLR2 and 5 protein expression in stimulated lymphocytes cells from subjects BS25 and BS26 by nutlin (10 µM) after 24 hr post-treatment. Proteins were determined by western blot analysis using antibodies specific to TLR2 and TLR5 in the membrane fractions. Actin protein provided a loading control. (While there is little change in the amount of actin protein detected, there may be differences in the nonspecific bands.) (B) Induction of TLR2 and 5 mRNA expression in stimulated lymphocytes from subjects BS25 and BS26 (data obtained from Figure 1) by nutlin.