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. 2011 Mar 31;7(3):e1001360. doi: 10.1371/journal.pgen.1001360

Figure 7. Induction of p53 sensitizes freshly isolated CD3+ cells to PAMP stimulation.

Figure 7

(A) TLR2 and cytokine (IL-1 and IL-8) expression in CD3+ lymphocytes incubated for 20 h with nutlin (10 µM) or DMSO and then exposed for 4 h to the TLR2 ligand PAM3CSK4 (1 µg/ml). Presented are representative results for subject BS#37. Gene expression was analyzed by qPCR and presented as fold-change compared to untreated cells. (B) Activation of p53 pathway by 24 h of nutlin treatment in CD3+ cells isolated from peripheral blood as determined by Western blot analysis.